ab 528428  (Developmental Studies Hybridoma Bank)

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Chodl mRNA is highly expressed in quiescent SCs (A) Chodl gene expression in various cell types in the mouse. Data were explored through the Tabula Muris data portal. (B) Relative expression of the Chodl gene in SCs, bladder cells, and a non-annotated cell population in (A). (C) UMAP-embedding of the scRNA-seq data on SCs isolated from non-injured muscles and injured muscles at 5 dpi in 2-month-old mice. (D) Violin plots show subcluster-specific gene expression ( Pax7 , Myog , Mki67 ) and enrichment of Chodl in the QSCs and ASCs. Colored by cluster identity. Abbreviations: QSC, quiescent SCs; SSC, self-renewal SCs; ASC, activated SCs; PSC, proliferating SCs; CSC, committed SCs; DSC, differentiating SCs. (E) qRT-PCR analysis of Chodl expression level in mouse QSC, ASC, and myoblast. n = 3 mice. (F) Immunoblot analysis showing CHODL expression in mouse myoblasts during myogenic differentiation. Relative band densitometry was calculated using ImageJ. (G) qRT-PCR analysis of Chodl in TA muscles post CTX injury in the mouse. n = 4 mice. Data are represented as mean ± SEM. The p values in E and G were analyzed using one-way ANOVA and Tukey post hoc test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Pax7 CreER/+ (stock #017763) Pax7 CreERT2(Gaka) (stock #017763), Rosa26-tdTomato LSL (stock #007909), and MyoD Cre/+ (stock #014140) mice were purchased from the Jackson Lab.

Techniques: Gene Expression, Expressing, Isolation, Muscles, Quantitative RT-PCR, Western Blot, Cell Characterization

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Loss of Chodl reduces SC number and delays muscle regeneration in young adult mice (A) Immunofluorescence of PAX7 and laminin on TA muscle cross-sections from young adult control (Ctrl) and Chodl MKO mice (2- to 3-month-old). White arrows indicate PAX7 + SCs. Scale bars, 50 μm. (B) Quantification of PAX7 + cells per area as shown in (A), n = 4 mice per group. (C) Schematics show experimental design involving cardiotoxin (CTX)-induced muscle regeneration in young adult mice. (D) TA muscle recovery rate calculated by the ratio of injured to uninjured TA muscle weight at 7 and 21 days post injury (dpi). n = 3 mice per group. (E) H&E staining of Ctrl and Chodl MKO mice TA muscle cross-sections at 7 and 21 dpi. Scale bars, 50 μm. (F) Immunofluorescence of laminin on Ctrl and Chodl MKO TA muscle cross-sections at 7 and 21 dpi. Scale bars, 50 μm. (G) Average myofiber cross-sectional area (CSA) of TA muscle cross-sections. n = 3 mice per group. (H) Immunofluorescence of PAX7 and laminin on Ctrl and Chodl MKO TA muscle cross-sections at 7 and 21 dpi. Scale bars, 50 μm. (I and J) Quantification of PAX7 + cell number per area at 7 (I) and 21 dpi (J). as shown in (H). For 7 dpi, n = 5 mice each group; For 21 dpi, n = 3 mice per group. Data are represented as mean ± SEM; unpaired Student’s t test; ∗ p < 0.05 and ∗∗ p < 0.01.

Article Snippet: Pax7 CreER/+ (stock #017763) Pax7 CreERT2(Gaka) (stock #017763), Rosa26-tdTomato LSL (stock #007909), and MyoD Cre/+ (stock #014140) mice were purchased from the Jackson Lab.

Techniques: Immunofluorescence, Control, Staining

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Chodl is necessary for SC maintenance and muscle regeneration in aging (A) Immunofluorescence of PAX7 and laminin on TA muscle cross-sections from old control (Ctrl) and Chodl MKO mice (20- to 22-month-old). White arrows indicate PAX7 + SCs. Scale bars, 50 μm. (B) Quantification of PAX7 + cells per area as shown in (A), n = 3 mice in each group. (C) Immunofluorescence of PAX7, laminin on freshly isolated myofibers from old Ctrl and Chodl MKO mice extensor digitorum longus (EDL) muscle. Scale bars, 20 μm. (D) Quantification of PAX7 + cells on myofibers as shown in (C). n = 3 mice, each group, 20–25 myofibers per mice. (E) Schematics show experimental design involving cardiotoxin (CTX)-induced muscle regeneration on old mice (20- to 22-month-old). (F) Representative images of TA muscles at 5 dpi in old Ctrl and Chodl MKO mice. (G) TA muscle recovery rate calculated by the ratio of injured to non-injured TA muscle weight in old Ctrl and Chodl MKO mice. Ctrl, n = 5 mice; Chodl MKO , n = 4 mice. (H) H&E staining of TA muscle cross-sections of old Ctrl and Chodl MKO mice at 5 dpi. Scale bars, 500 μm for the upper panel, 50 μm for the bottom panel. (I and J) Relative frequency of myofiber cross-sectional area (CSA) (I) and dot plot of average myofiber CSA (J). n = 4 mice in each group. (K and L) Immunofluorescence of PAX7, laminin (K), and quantification of PAX7 + cell number (L) on TA muscle cross-sections. Ctrl, n = 5 mice; Chodl MKO , n = 4 mice. Scale bars, 50 μm. (M and N) Immunofluorescence of MyoG, laminin (M), and quantification of MyoG + cell number (N) on TA muscle cross-sections. Ctrl, n = 5 mice; Chodl MKO , n = 4 mice. Scale bars, 50 μm. Data are represented as mean ± SEM; Student’s t test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Pax7 CreER/+ (stock #017763) Pax7 CreERT2(Gaka) (stock #017763), Rosa26-tdTomato LSL (stock #007909), and MyoD Cre/+ (stock #014140) mice were purchased from the Jackson Lab.

Techniques: Immunofluorescence, Control, Isolation, Muscles, Staining

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Inducible deletion of Chodl in adult SCs decreases proliferation and impedes muscle regeneration (A) Schematics show CTX injury and EdU labeling of cell proliferation in 2-month-old control (Ctrl) and Chodl PKO mice after tamoxifen (TMX)-induced specific knockout of Chodl in SCs. (B) Immunofluorescence of eMyHC on TA muscle cross-sections. Scale bars, 50 μm. (C) Quantification of the eMyHC + cell number per area as shown in (B). (D) Immunofluorescence of PAX7 and laminin on TA muscle cross-sections. Scale bars, 50 μm. (E) Quantification of the number of PAX7+ cells per area, as shown in (D). (F) Immunofluorescence of PAX7, laminin, and EdU on TA muscle cross-sections. Scale bars, 50 μm. (G) Quantification of the average number of PAX7 + /EdU + and PAX7 + cells per area, as shown in (F). Data are represented as mean ± SEM; unpaired Student’s t test; ∗ p < 0.05 and ∗∗ p < 0.01.

Article Snippet: Pax7 CreER/+ (stock #017763) Pax7 CreERT2(Gaka) (stock #017763), Rosa26-tdTomato LSL (stock #007909), and MyoD Cre/+ (stock #014140) mice were purchased from the Jackson Lab.

Techniques: Labeling, Control, Knock-Out, Immunofluorescence

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Chodl is critical for retaining SCs in the myofiber niche during development (A) Immunofluorescence of PAX7, laminin on freshly isolated myofibers from young adult (2-month-old) control (Ctrl) and Chodl PKO mice extensor digitorum longus (EDL) muscles. Scale bars, 20 μm. (B) Percentage of satellite cells (SCs) detached from the fiber as shown in (A). n = 4 mice per group, 20–25 fibers per mice. (C) Immunofluorescence of PAX7, laminin on TA muscle cross-sections of adult Ctrl and Chodl PKO mice. Scale bars, 20 μm for the left three panels, 10 μm for the right panel with enlarged area. (D) Percentage of SCs localized at the interstitial space as shown in (C), n = 4 mice per group. (E) Immunofluorescence of PAX7, EdU on TA muscle cross-sections of young adult Ctrl and Chodl PKO mice. Red arrowhead indicates EdU/PAX7 double-positive cells. Scale bars, 20 μm. (F) Percentage of EdU/PAX7 double-positive cells to total PAX7 + cells as shown in (E), n = 4 mice per group. (G) Immunofluorescence of PAX7 and laminin on TA muscle cross-sections of Ctrl and Chodl MKO mice at postnatal day 7 (P7). Scale bars, 20 μm. (H) Percentage of SCs localized at the interstitial space as shown in (G), n = 4 mice per group. (I) Immunofluorescence of PAX7, laminin on TA muscle cross-sections of Ctrl and Chodl MKO mice at postnatal day 21 (P21). Scale bars, 20 μm. (J) Percentage of SCs localized at the interstitial space as shown in (J), n = 4 mice per group. Data are represented as mean ± SEM; unpaired Student’s t test; ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

Article Snippet: Pax7 CreER/+ (stock #017763) Pax7 CreERT2(Gaka) (stock #017763), Rosa26-tdTomato LSL (stock #007909), and MyoD Cre/+ (stock #014140) mice were purchased from the Jackson Lab.

Techniques: Immunofluorescence, Isolation, Control, Muscles

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Chodl mRNA is highly expressed in quiescent SCs (A) Chodl gene expression in various cell types in the mouse. Data were explored through the Tabula Muris data portal. (B) Relative expression of the Chodl gene in SCs, bladder cells, and a non-annotated cell population in (A). (C) UMAP-embedding of the scRNA-seq data on SCs isolated from non-injured muscles and injured muscles at 5 dpi in 2-month-old mice. (D) Violin plots show subcluster-specific gene expression ( Pax7 , Myog , Mki67 ) and enrichment of Chodl in the QSCs and ASCs. Colored by cluster identity. Abbreviations: QSC, quiescent SCs; SSC, self-renewal SCs; ASC, activated SCs; PSC, proliferating SCs; CSC, committed SCs; DSC, differentiating SCs. (E) qRT-PCR analysis of Chodl expression level in mouse QSC, ASC, and myoblast. n = 3 mice. (F) Immunoblot analysis showing CHODL expression in mouse myoblasts during myogenic differentiation. Relative band densitometry was calculated using ImageJ. (G) qRT-PCR analysis of Chodl in TA muscles post CTX injury in the mouse. n = 4 mice. Data are represented as mean ± SEM. The p values in E and G were analyzed using one-way ANOVA and Tukey post hoc test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Mouse: B6;129 -Pax7 tm2.1(cre/ERT2)Fan /J , The Jackson Laboratory , JAX stock: #012476.

Techniques: Gene Expression, Expressing, Isolation, Muscles, Quantitative RT-PCR, Western Blot, Cell Characterization

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Loss of Chodl reduces SC number and delays muscle regeneration in young adult mice (A) Immunofluorescence of PAX7 and laminin on TA muscle cross-sections from young adult control (Ctrl) and Chodl MKO mice (2- to 3-month-old). White arrows indicate PAX7 + SCs. Scale bars, 50 μm. (B) Quantification of PAX7 + cells per area as shown in (A), n = 4 mice per group. (C) Schematics show experimental design involving cardiotoxin (CTX)-induced muscle regeneration in young adult mice. (D) TA muscle recovery rate calculated by the ratio of injured to uninjured TA muscle weight at 7 and 21 days post injury (dpi). n = 3 mice per group. (E) H&E staining of Ctrl and Chodl MKO mice TA muscle cross-sections at 7 and 21 dpi. Scale bars, 50 μm. (F) Immunofluorescence of laminin on Ctrl and Chodl MKO TA muscle cross-sections at 7 and 21 dpi. Scale bars, 50 μm. (G) Average myofiber cross-sectional area (CSA) of TA muscle cross-sections. n = 3 mice per group. (H) Immunofluorescence of PAX7 and laminin on Ctrl and Chodl MKO TA muscle cross-sections at 7 and 21 dpi. Scale bars, 50 μm. (I and J) Quantification of PAX7 + cell number per area at 7 (I) and 21 dpi (J). as shown in (H). For 7 dpi, n = 5 mice each group; For 21 dpi, n = 3 mice per group. Data are represented as mean ± SEM; unpaired Student’s t test; ∗ p < 0.05 and ∗∗ p < 0.01.

Article Snippet: Mouse: B6;129 -Pax7 tm2.1(cre/ERT2)Fan /J , The Jackson Laboratory , JAX stock: #012476.

Techniques: Immunofluorescence, Control, Staining

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Chodl is necessary for SC maintenance and muscle regeneration in aging (A) Immunofluorescence of PAX7 and laminin on TA muscle cross-sections from old control (Ctrl) and Chodl MKO mice (20- to 22-month-old). White arrows indicate PAX7 + SCs. Scale bars, 50 μm. (B) Quantification of PAX7 + cells per area as shown in (A), n = 3 mice in each group. (C) Immunofluorescence of PAX7, laminin on freshly isolated myofibers from old Ctrl and Chodl MKO mice extensor digitorum longus (EDL) muscle. Scale bars, 20 μm. (D) Quantification of PAX7 + cells on myofibers as shown in (C). n = 3 mice, each group, 20–25 myofibers per mice. (E) Schematics show experimental design involving cardiotoxin (CTX)-induced muscle regeneration on old mice (20- to 22-month-old). (F) Representative images of TA muscles at 5 dpi in old Ctrl and Chodl MKO mice. (G) TA muscle recovery rate calculated by the ratio of injured to non-injured TA muscle weight in old Ctrl and Chodl MKO mice. Ctrl, n = 5 mice; Chodl MKO , n = 4 mice. (H) H&E staining of TA muscle cross-sections of old Ctrl and Chodl MKO mice at 5 dpi. Scale bars, 500 μm for the upper panel, 50 μm for the bottom panel. (I and J) Relative frequency of myofiber cross-sectional area (CSA) (I) and dot plot of average myofiber CSA (J). n = 4 mice in each group. (K and L) Immunofluorescence of PAX7, laminin (K), and quantification of PAX7 + cell number (L) on TA muscle cross-sections. Ctrl, n = 5 mice; Chodl MKO , n = 4 mice. Scale bars, 50 μm. (M and N) Immunofluorescence of MyoG, laminin (M), and quantification of MyoG + cell number (N) on TA muscle cross-sections. Ctrl, n = 5 mice; Chodl MKO , n = 4 mice. Scale bars, 50 μm. Data are represented as mean ± SEM; Student’s t test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Mouse: B6;129 -Pax7 tm2.1(cre/ERT2)Fan /J , The Jackson Laboratory , JAX stock: #012476.

Techniques: Immunofluorescence, Control, Isolation, Muscles, Staining

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Inducible deletion of Chodl in adult SCs decreases proliferation and impedes muscle regeneration (A) Schematics show CTX injury and EdU labeling of cell proliferation in 2-month-old control (Ctrl) and Chodl PKO mice after tamoxifen (TMX)-induced specific knockout of Chodl in SCs. (B) Immunofluorescence of eMyHC on TA muscle cross-sections. Scale bars, 50 μm. (C) Quantification of the eMyHC + cell number per area as shown in (B). (D) Immunofluorescence of PAX7 and laminin on TA muscle cross-sections. Scale bars, 50 μm. (E) Quantification of the number of PAX7+ cells per area, as shown in (D). (F) Immunofluorescence of PAX7, laminin, and EdU on TA muscle cross-sections. Scale bars, 50 μm. (G) Quantification of the average number of PAX7 + /EdU + and PAX7 + cells per area, as shown in (F). Data are represented as mean ± SEM; unpaired Student’s t test; ∗ p < 0.05 and ∗∗ p < 0.01.

Article Snippet: Mouse: B6;129 -Pax7 tm2.1(cre/ERT2)Fan /J , The Jackson Laboratory , JAX stock: #012476.

Techniques: Labeling, Control, Knock-Out, Immunofluorescence

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Chodl is critical for retaining SCs in the myofiber niche during development (A) Immunofluorescence of PAX7, laminin on freshly isolated myofibers from young adult (2-month-old) control (Ctrl) and Chodl PKO mice extensor digitorum longus (EDL) muscles. Scale bars, 20 μm. (B) Percentage of satellite cells (SCs) detached from the fiber as shown in (A). n = 4 mice per group, 20–25 fibers per mice. (C) Immunofluorescence of PAX7, laminin on TA muscle cross-sections of adult Ctrl and Chodl PKO mice. Scale bars, 20 μm for the left three panels, 10 μm for the right panel with enlarged area. (D) Percentage of SCs localized at the interstitial space as shown in (C), n = 4 mice per group. (E) Immunofluorescence of PAX7, EdU on TA muscle cross-sections of young adult Ctrl and Chodl PKO mice. Red arrowhead indicates EdU/PAX7 double-positive cells. Scale bars, 20 μm. (F) Percentage of EdU/PAX7 double-positive cells to total PAX7 + cells as shown in (E), n = 4 mice per group. (G) Immunofluorescence of PAX7 and laminin on TA muscle cross-sections of Ctrl and Chodl MKO mice at postnatal day 7 (P7). Scale bars, 20 μm. (H) Percentage of SCs localized at the interstitial space as shown in (G), n = 4 mice per group. (I) Immunofluorescence of PAX7, laminin on TA muscle cross-sections of Ctrl and Chodl MKO mice at postnatal day 21 (P21). Scale bars, 20 μm. (J) Percentage of SCs localized at the interstitial space as shown in (J), n = 4 mice per group. Data are represented as mean ± SEM; unpaired Student’s t test; ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

Article Snippet: Mouse: B6;129 -Pax7 tm2.1(cre/ERT2)Fan /J , The Jackson Laboratory , JAX stock: #012476.

Techniques: Immunofluorescence, Isolation, Control, Muscles

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Chodl mRNA is highly expressed in quiescent SCs (A) Chodl gene expression in various cell types in the mouse. Data were explored through the Tabula Muris data portal. (B) Relative expression of the Chodl gene in SCs, bladder cells, and a non-annotated cell population in (A). (C) UMAP-embedding of the scRNA-seq data on SCs isolated from non-injured muscles and injured muscles at 5 dpi in 2-month-old mice. (D) Violin plots show subcluster-specific gene expression ( Pax7 , Myog , Mki67 ) and enrichment of Chodl in the QSCs and ASCs. Colored by cluster identity. Abbreviations: QSC, quiescent SCs; SSC, self-renewal SCs; ASC, activated SCs; PSC, proliferating SCs; CSC, committed SCs; DSC, differentiating SCs. (E) qRT-PCR analysis of Chodl expression level in mouse QSC, ASC, and myoblast. n = 3 mice. (F) Immunoblot analysis showing CHODL expression in mouse myoblasts during myogenic differentiation. Relative band densitometry was calculated using ImageJ. (G) qRT-PCR analysis of Chodl in TA muscles post CTX injury in the mouse. n = 4 mice. Data are represented as mean ± SEM. The p values in E and G were analyzed using one-way ANOVA and Tukey post hoc test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Mouse: B6.Cg- Pax7 tm1(cre/ERT2)Gaka /J , The Jackson Laboratory , JAX stock: #017763.

Techniques: Gene Expression, Expressing, Isolation, Muscles, Quantitative RT-PCR, Western Blot, Cell Characterization

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Loss of Chodl reduces SC number and delays muscle regeneration in young adult mice (A) Immunofluorescence of PAX7 and laminin on TA muscle cross-sections from young adult control (Ctrl) and Chodl MKO mice (2- to 3-month-old). White arrows indicate PAX7 + SCs. Scale bars, 50 μm. (B) Quantification of PAX7 + cells per area as shown in (A), n = 4 mice per group. (C) Schematics show experimental design involving cardiotoxin (CTX)-induced muscle regeneration in young adult mice. (D) TA muscle recovery rate calculated by the ratio of injured to uninjured TA muscle weight at 7 and 21 days post injury (dpi). n = 3 mice per group. (E) H&E staining of Ctrl and Chodl MKO mice TA muscle cross-sections at 7 and 21 dpi. Scale bars, 50 μm. (F) Immunofluorescence of laminin on Ctrl and Chodl MKO TA muscle cross-sections at 7 and 21 dpi. Scale bars, 50 μm. (G) Average myofiber cross-sectional area (CSA) of TA muscle cross-sections. n = 3 mice per group. (H) Immunofluorescence of PAX7 and laminin on Ctrl and Chodl MKO TA muscle cross-sections at 7 and 21 dpi. Scale bars, 50 μm. (I and J) Quantification of PAX7 + cell number per area at 7 (I) and 21 dpi (J). as shown in (H). For 7 dpi, n = 5 mice each group; For 21 dpi, n = 3 mice per group. Data are represented as mean ± SEM; unpaired Student’s t test; ∗ p < 0.05 and ∗∗ p < 0.01.

Article Snippet: Mouse: B6.Cg- Pax7 tm1(cre/ERT2)Gaka /J , The Jackson Laboratory , JAX stock: #017763.

Techniques: Immunofluorescence, Control, Staining

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Chodl is necessary for SC maintenance and muscle regeneration in aging (A) Immunofluorescence of PAX7 and laminin on TA muscle cross-sections from old control (Ctrl) and Chodl MKO mice (20- to 22-month-old). White arrows indicate PAX7 + SCs. Scale bars, 50 μm. (B) Quantification of PAX7 + cells per area as shown in (A), n = 3 mice in each group. (C) Immunofluorescence of PAX7, laminin on freshly isolated myofibers from old Ctrl and Chodl MKO mice extensor digitorum longus (EDL) muscle. Scale bars, 20 μm. (D) Quantification of PAX7 + cells on myofibers as shown in (C). n = 3 mice, each group, 20–25 myofibers per mice. (E) Schematics show experimental design involving cardiotoxin (CTX)-induced muscle regeneration on old mice (20- to 22-month-old). (F) Representative images of TA muscles at 5 dpi in old Ctrl and Chodl MKO mice. (G) TA muscle recovery rate calculated by the ratio of injured to non-injured TA muscle weight in old Ctrl and Chodl MKO mice. Ctrl, n = 5 mice; Chodl MKO , n = 4 mice. (H) H&E staining of TA muscle cross-sections of old Ctrl and Chodl MKO mice at 5 dpi. Scale bars, 500 μm for the upper panel, 50 μm for the bottom panel. (I and J) Relative frequency of myofiber cross-sectional area (CSA) (I) and dot plot of average myofiber CSA (J). n = 4 mice in each group. (K and L) Immunofluorescence of PAX7, laminin (K), and quantification of PAX7 + cell number (L) on TA muscle cross-sections. Ctrl, n = 5 mice; Chodl MKO , n = 4 mice. Scale bars, 50 μm. (M and N) Immunofluorescence of MyoG, laminin (M), and quantification of MyoG + cell number (N) on TA muscle cross-sections. Ctrl, n = 5 mice; Chodl MKO , n = 4 mice. Scale bars, 50 μm. Data are represented as mean ± SEM; Student’s t test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Mouse: B6.Cg- Pax7 tm1(cre/ERT2)Gaka /J , The Jackson Laboratory , JAX stock: #017763.

Techniques: Immunofluorescence, Control, Isolation, Muscles, Staining

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Inducible deletion of Chodl in adult SCs decreases proliferation and impedes muscle regeneration (A) Schematics show CTX injury and EdU labeling of cell proliferation in 2-month-old control (Ctrl) and Chodl PKO mice after tamoxifen (TMX)-induced specific knockout of Chodl in SCs. (B) Immunofluorescence of eMyHC on TA muscle cross-sections. Scale bars, 50 μm. (C) Quantification of the eMyHC + cell number per area as shown in (B). (D) Immunofluorescence of PAX7 and laminin on TA muscle cross-sections. Scale bars, 50 μm. (E) Quantification of the number of PAX7+ cells per area, as shown in (D). (F) Immunofluorescence of PAX7, laminin, and EdU on TA muscle cross-sections. Scale bars, 50 μm. (G) Quantification of the average number of PAX7 + /EdU + and PAX7 + cells per area, as shown in (F). Data are represented as mean ± SEM; unpaired Student’s t test; ∗ p < 0.05 and ∗∗ p < 0.01.

Article Snippet: Mouse: B6.Cg- Pax7 tm1(cre/ERT2)Gaka /J , The Jackson Laboratory , JAX stock: #017763.

Techniques: Labeling, Control, Knock-Out, Immunofluorescence

Journal: iScience

Article Title: Chondrolectin regulates the sublaminar localization and regenerative function of muscle satellite cells in mice

doi: 10.1016/j.isci.2026.115703

Figure Lengend Snippet: Chodl is critical for retaining SCs in the myofiber niche during development (A) Immunofluorescence of PAX7, laminin on freshly isolated myofibers from young adult (2-month-old) control (Ctrl) and Chodl PKO mice extensor digitorum longus (EDL) muscles. Scale bars, 20 μm. (B) Percentage of satellite cells (SCs) detached from the fiber as shown in (A). n = 4 mice per group, 20–25 fibers per mice. (C) Immunofluorescence of PAX7, laminin on TA muscle cross-sections of adult Ctrl and Chodl PKO mice. Scale bars, 20 μm for the left three panels, 10 μm for the right panel with enlarged area. (D) Percentage of SCs localized at the interstitial space as shown in (C), n = 4 mice per group. (E) Immunofluorescence of PAX7, EdU on TA muscle cross-sections of young adult Ctrl and Chodl PKO mice. Red arrowhead indicates EdU/PAX7 double-positive cells. Scale bars, 20 μm. (F) Percentage of EdU/PAX7 double-positive cells to total PAX7 + cells as shown in (E), n = 4 mice per group. (G) Immunofluorescence of PAX7 and laminin on TA muscle cross-sections of Ctrl and Chodl MKO mice at postnatal day 7 (P7). Scale bars, 20 μm. (H) Percentage of SCs localized at the interstitial space as shown in (G), n = 4 mice per group. (I) Immunofluorescence of PAX7, laminin on TA muscle cross-sections of Ctrl and Chodl MKO mice at postnatal day 21 (P21). Scale bars, 20 μm. (J) Percentage of SCs localized at the interstitial space as shown in (J), n = 4 mice per group. Data are represented as mean ± SEM; unpaired Student’s t test; ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

Article Snippet: Mouse: B6.Cg- Pax7 tm1(cre/ERT2)Gaka /J , The Jackson Laboratory , JAX stock: #017763.

Techniques: Immunofluorescence, Isolation, Control, Muscles

Journal: Journal of Tissue Engineering

Article Title: Fibro-adipogenic progenitors enhance functional and structural properties of human 3D tissue engineered skeletal muscles

doi: 10.1177/20417314261441552

Figure Lengend Snippet: Characterization of MPs, iFAPs and co-cultures in 2D: (a) MP monolayer culture after 3 days of myogenic differentiation, stained for desmin, TE-7 and PAX7 and the associated quantifications ( N = 16, independent stained wells), (b) graphical overview of FAP isolation, expansion and immortalization. (c) MP versus iFAP monolayer cultures after 2 days of proliferation, with multiple ECM protein immunodetections, (d) MP versus iFAP monolayer cultures after 5 days of fibrogenic differentiation, with multiple ECM protein immunodetections, (e) MP and iFAP monolayer cultures and co-cultures after myo-, fibro- or adipogenic differentiation and their respective immunodetections. Bar graphs depict quantifications of MF20, Collagen I and Oil Red O. Statistical significance was assessed using a one-way ANOVA, followed by Tukey correction for multiple comparisons, * p < 0.05, **** p < 0.0001. Error bars represent standard deviations. N = 8, depicting independently stained wells, (f) double lineage differentiation of iFAPs after fibro- and adipogenic media was added, immunodetections with collagen I and perilipin, and (g) triple differentiation of a co-culture, resulting in myotubes, fibroblasts and adipocytes, immunodetections with MF20 (recognizes all myosin heavy chain isoforms), collagen I and perilipin.

Article Snippet: PAX7 , Mouse , 1:50 , DSHB , PAX7.

Techniques: Cell Characterization, Staining, Isolation, Co-Culture Assay